Genetic Code: Introducing Pyrrolysine
نویسندگان
چکیده
منابع مشابه
Genetic Code: Introducing Pyrrolysine
Monomethylamine methyltransferase of the archaebacterium Methanosarcina barkeri contains a novel amino acid, pyrrolysine, encoded by the termination codon UAG. Initial studies suggest that pyrrolysine may be co-translationally inserted during protein synthesis, probably by a mechanism analogous to that operating during selenocysteine incorporation.
متن کاملAdding pyrrolysine to the Escherichia coli genetic code.
Pyrrolysyl-tRNA synthetase and its cognate suppressor tRNA(Pyl) mediate pyrrolysine (Pyl) insertion at in frame UAG codons. The presence of an RNA hairpin structure named Pyl insertion structure (PYLIS) downstream of the suppression site has been shown to stimulate the insertion of Pyl in archaea. We study here the impact of the presence of PYLIS on the level of Pyl and the Pyl analog N-epsilon...
متن کاملA natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine.
Pyrrolysine has entered natural genetic codes by the translation of UAG, a canonical stop codon. UAG translation as pyrrolysine requires the pylT gene product, an amber-decoding tRNA(Pyl) that is aminoacylated with pyrrolysine by the pyrrolysyl-tRNA synthetase produced from the pylS gene. The pylTS genes form a gene cluster with pylBCD, whose functions have not been investigated. The pylTSBCD g...
متن کاملReducing Pyrrolysine tRNA Copy Number Improves the Performance of Genetic Code Expansion in Live Cell Imaging of Bioorthogonally Labeled Proteins
Genetic code expansion technology enables the incorporation of non-canonical amino acids (NCAAs) into proteins expressed in live cells. The NCAA is commonly encoded by an in-frame amber stop codon (TAG) and the methodology relies on the use of an orthogonal aminoacyl tRNA synthetase and its cognate amber suppressor tRNA; e.g., the pyrrolysine synthetase / tRNA CUA (PylT) pair. It is widely acce...
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Genetic psychophysiology examines interindividual variation in psychophysiological traits using behavioral genetic and molecular genetic techniques. It aims to delineate the pathways that lead from genomic variation to individual differences in cognitive abilities, affect regulation, and mental and physical health. This editorial provides an introduction to the twin design and gene finding stra...
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ژورنال
عنوان ژورنال: Current Biology
سال: 2002
ISSN: 0960-9822
DOI: 10.1016/s0960-9822(02)00947-8